Preparation and Characterization of Quercetin Phytosome

ABSTRACTThe goal of this work is to improve quercetin's solubility and bioavailability by creating and characterizing quercetinphytosomes using the solvent evaporation technique. After obtaining phospholipids from ghee leftovers a quercetin-phosphatidylcholine complex was synthesized and its physicochemical characteristics were assessed. Analytical tools suchas Differential Scanning Calorimetry (DSC), Fourier Transform Infrared Spectroscopy (IR), X-ray Diffraction (XRD),Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM) were used to conduct thecharacterization. Quercetin and phosphatidylcholine have formed a stable combination, as shown by DSC and IR studies.XRD, on the other hand, showed a change to a less crystalline state, suggesting improved solubility. SEM and TEM imagesshowed uniform, nanoscale spherical particles, supporting the successful encapsulation of quercetin. Thus, the 1:2 ratio isidentified as the optimal formulation for maximizing practical yield, underscoring the importance of phospholipidconcentration in achieving higher efficiency. The results indicate that the phytosome formulation enhances the stability,solubility, and bioavailability of quercetin, providing a promising strategy for its therapeutic applications