Lactiplantibacillus plantarumOLL2712 Protects From the Intestinal Dysfunction in D‐Galactose Induced Senescent Cells

Cellular senescence refers to a state in which cells stop dividing and can no longer proliferate because of various factors, such as DNA damage and oxidative stress. It is implicated as a key driver of aging and age‐related diseases. Lactiplantibacillus plantarum OLL2712 (OLL2712) is a lactic acid bacterium that alleviates chronic inflammation and improves the intestinal barrier and cognitive functions. These effects are important for preventing the changes that occur in the body with aging. In this study, we hypothesized that OLL2712 protects against intestinal barrier dysfunction associated with cellular senescence. To reveal the antiaging effect of OLL2712, we used D‐galactose (D‐gal) to induce cellular senescence in Caco‐2 cells and investigated its effects on various phenomena occurring in senescent intestinal cells. The results showed that OLL2712 suppressed D‐gal‐induced cellular senescence and intestinal barrier dysfunction. Furthermore, microarray analysis showed that OLL2712 upregulated signaling involved in antioxidant effects, metabolism, and cell division, which were downregulated by cellular senescence. Overall, OLL2712 maintained intracellular homeostasis and restored intestinal barrier dysfunction associated with cellular senescence. We discovered the possibility that paraprobiotics contribute to the antiaging effects.