Synthesis, Duplex-forming Properties, and Enzymatic Resistance of Oligonucleotides Containing N-acylsulfonamide Linkages

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This is a Quantitative Study study.

Romain Amador; Carine Baraguey; Guillaume Clavé; Michaël Smietana

doi:10.1039/d5ob01382h

January 1, 2025Organic & Biomolecular Chemistry

Synthesis, Duplex-forming Properties, and Enzymatic Resistance of Oligonucleotides Containing N-acylsulfonamide Linkages

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Key Points

The NAC linkage enhances the thermal stability of oligonucleotides, providing insights on enzymatic resistance.
Thermal denaturation studies indicate that oligonucleotides containing NAC linkages exhibit improved duplex stability.
The study utilized the sulfo-click reaction to synthesize the NAC-linked thymidine dimer for oligonucleotide incorporation.
Findings suggest potential applications for oligonucleotides containing N-acylsulfonamide linkages in therapeutic contexts.

Implication

We report synthesis and properties of DNA oligonucleotides with N-acylsulfonamide linkages, showing enhanced thermal stability.

Abstract

We report here the synthesis of (2-(N-acetylsulfamoyl)ethyl)carbamate (NAC)-linked thymidine dimer via the sulfo-click reaction and its incorporation into several DNA oligonucleotides. Thermal denaturation studies revealed that the NAC linkage exhibits...

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Cite This Study

Amador et al. (2025) studied this question.

synapsesocial.com/papers/68dc261d8a7d58c25ebb2a7b https://doi.org/10.1039/d5ob01382h

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Synthesis, Duplex-forming Properties, and Enzymatic Resistance of Oligonucleotides Containing N-acylsulfonamide Linkages

Key Points

Implication

Abstract

Discussion

Cite This Study

Synthesis, Duplex-forming Properties, and Enzymatic Resistance of Oligonucleotides Containing N-acylsulfonamide Linkages

Key Points

Implication

Abstract

Discussion

Cite This Study

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