⌘+K

Pulse Explore Communities Researchers Journals

Browser extensionInstagram

Home Communities Explore

February 23, 2022

DAB precipitation by Laccase Oxidation v1

Song-Yi Lee Heegwang Roh Mason Mackey Alice Y. Ting Mark H. Ellisman

View at Publisher

Ask AI

Bookmark

Share

Key Points

LaccID successfully precipitated DAB without hydrogen peroxide or light, demonstrating an efficient enzymatic process.
HEX293T cells expressing LaccID allowed for effective DAB precipitation observed via transmission electron microscopy (TEM).
This approach may enhance biocatalytic applications by eliminating the need for external oxidants or light sources.
The findings offer potential advancements in biochemistry and synthetic biology using engineered enzymes.

Ask about this paper

Answers are grounded in this paper and cite their sources.

Ask your own question…

Implication

Oxidase LaccID enables DAB precipitation in HEX293T cells, indicating a novel approach for biocatalysis.

Abstract

No abstract yet. The journal hasn't published one we can show.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Lee et al. (2022) studied this question.

synapsesocial.com/papers/68de70f583cbc991d0a23645 https://doi.org/10.17504/protocols.io.bp2l6195zvqe/v1

View at Publisher

Ask AI

Bookmark

Share

Keep reading

Synapse has enriched 8 closely related papers on similar clinical questions. Consider them for comparative context:

1Directed evolution of LaccID for cell surface proximity labeling and electron microscopyOriginal Research · 2025 · 37 citations
2Labeling and DAB oxidation of EdU-treated HEK293T cells with Cy5 azide and Fe-TAML azide for light and transmission electron microscopy v12024
3DAB Immunohistochemistry (IHC) Staining for Stereological Analysis v12024
4Achieving efficient photo-enzyme coupling via a novel diketone derivative to expand the scope of enzyme actionOriginal Research · 2025
5Detoxification of tetracycline and synthetic dyes by a newly characterized Lentinula edodes laccase, and safety assessment using proteomic analysis2024 · 19 citations
6Immobilization of Laccase in β-Cyclodextrin Composite Hydrogel for Efficient Degradation of Dye Pollutants2024 · 2 citations
7Computational Insights into Enzyme‐Substrate Binding Interplay Exhibit Variable Binding Attributes: A Framework for Implementing Oxidoreductase‐Based Applications2024 · 2 citations
8Purification and Characterization of a Novel Laccase from Cerrena sp. HYB07 with Dye Decolorizing AbilityOriginal Research · 2014 · 87 citations