Labeling and DAB oxidation of EdU-treated HEK293T cells with Cy5 azide and Fe-TAML azide for light and transmission electron microscopy v1

Stephen Adams; Mason Mackey; Mark H. Ellisman

doi:10.17504/protocols.io.n2bvjn8jxgk5/v1

May 16, 2024Open Access

Labeling and DAB oxidation of EdU-treated HEK293T cells with Cy5 azide and Fe-TAML azide for light and transmission electron microscopy v1

View Full Paper

Ask AI

Bookmark

Share

Ask about this paper

Answers are grounded in this paper and cite their sources.

Abstract

This protocol outlines the click chemistry labeling of EdU-pulsed DNA with Fe-TAML azide to catalyze DAB oxidation by hydrogen peroxide to generate localized osmiophilic precipitate detectable by light and electron microscopy.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Adams et al. (2024) studied this question.

synapsesocial.com/papers/68e69d5db6db643587622d7f https://doi.org/10.17504/protocols.io.n2bvjn8jxgk5/v1

View Full Paper

Ask AI

Bookmark

Share

Labeling and DAB oxidation of EdU-treated HEK293T cells with Cy5 azide and Fe-TAML azide for light and transmission electron microscopy v1

Ask about this paper

Abstract

Discussion

Cite This Study

Labeling and DAB oxidation of EdU-treated HEK293T cells with Cy5 azide and Fe-TAML azide for light and transmission electron microscopy v1

Ask about this paper

Abstract

Discussion

Cite This Study

Keep reading

Keep reading