From Rh Blood Group Antigen Antibody Testing to “Transfusionomics”

Objective To analyze the results of Rh blood group antigen detection and irregular antibody identification of 5050 patients in China, and to explore the importance of Rh blood group typing before blood transfusion and to propose a new concept of “Transfusionomics”. Methods Use microcolumn gel detection card to type Rh blood group antigen of 5050 patients, and to screen and identify antibody. Use PCR amplification and Sanger sequencing techniques to detect RHD genotype. Results A total of 5030 D‐positive cases were detected in 5050 patients; the remaining 20 cases were detected as D‐negative. Totally, 49 cases of Rh blood group system antibodies were detected, which accounted for 70% of all detected antibodies. Of the 20 initial screening D‐negative patients, 11 cases had RHD gene deletion, and 9 cases had retained all of 10 exons. Conclusion Large‐scale testing and research on patients' Rh blood group antigen and antibody is aimed at avoiding red blood cell alloimmunization caused by blood transfusion, realizing individualized and precise blood transfusion. The large amount of data generated by blood transfusion laboratories every day will form an important basis for a new concept of “Transfusionomics”.