What question did this study set out to answer?

To identify and genetically characterize novel RHD variant alleles among serologically RH1-negative first-time blood donors identified via routine molecular screening in Switzerland.

August 14, 2026Transfusion

Three novel RHD variant alleles were identified through routine molecular screening of serologically RH1 negative first‐time blood donors

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Authors

KRKräuchi RahelSwiss Red Cross BSBernd SchimanskiSwiss Red Cross TCTinguely CarolineSwiss Red Cross

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Implication

Molecular screening study reveals three novel RHD variant alleles in serologically RH1-negative first-time blood donors, highlighting the need for genetic testing to prevent alloimmunization.

Key Points

To identify and genetically characterize novel RHD variant alleles among serologically RH1-negative first-time blood donors identified via routine molecular screening in Switzerland.
Performed serological RH phenotyping using column agglutination, adsorption-elution testing, and monoclonal antibody panels.
Extracted genomic DNA and RNA from first-time blood donors to conduct SSP-PCR, RHD exon sequencing, and cDNA sequencing.
Identified a nonsense mutation (c.265C>T; p.Gln89Ter) in two donors presenting with an apparent RH1-negative phenotype but weakly positive adsorption-elution results.
Characterized a missense mutation (c.863T>A; p.Ile288Asn) in one donor associated with extremely weak RH1 antigen expression.
Identified a frameshift mutation (c.970del; p.His324Thrfs*35) leading to predicted protein truncation and a true RH1-negative phenotype in one donor.

Cite This Study

Rahel et al. (2026) studied this question.

synapsesocial.com/papers/6a7ec754b70b84ec8b9138d6 https://doi.org/10.1111/trf.70352

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Implication

Molecular screening study reveals three novel RHD variant alleles in serologically RH1-negative first-time blood donors, highlighting the need for genetic testing to prevent alloimmunization.

Three novel RHD variant alleles were identified through routine molecular screening of serologically RH1 negative first‐time blood donors

Discussion

Member takes

Implication

Key Points

Cite This Study

Three novel RHD variant alleles were identified through routine molecular screening of serologically RH1 negative first‐time blood donors

Discussion

Member takes

Implication

Key Points

Cite This Study

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