Supplementary Fig. S10 from Perivascular NOTCH3⁺ Stem Cells Drive Meningioma Tumorigenesis and Resistance to Radiotherapy

Supplementary Fig. 10. NOTCH3 is expressed in mural cells during brain vascular development and homeostasis. A, IF for NOTCH3, the mural cell marker PDGFR, and the endothelial cell marker CD34 in the developing human cortex from gestational week 17. NOTCH3 colocalizes with PDGFR in the marginal zone, which contributes to meningeal development (42–45). Representative of n=3 biological replicates. DAPI marks DNA. Unperfused red blood cells in the vascular lumen are autofluorescent in the NOTCH3 channel. Scale bars, 100µm (top), 10µm (bottom), and 1µm (insert). B, Single-cell RNA sequencing cell cluster UMAP and feature plots from re-analysis of 139,134 perinatal human brain vasculature transcriptomes (28) showing NOTCH3 is enriched in developing mural cells marked by ACTA2 or PDGFRB but not in developing endothelia marked by PECAM1. Samples for single-cell RNA sequencing were obtained from gestational weeks 15, 17, 18, 20, 22, and 23. C, Single-cell RNA sequencing cell cluster UMAP and feature plots from re-analysis of 84,138 adult human brain vasculature transcriptomes (30) showing NOTCH3 is expressed in mural cell lineages (pericytes, smooth muscle cells) but not endothelial cell lineages (venous cells, arterial cells, capillary cells). D, Single-cell RNA sequencing cell cluster UMAP and feature plots from re-analysis of 52,023 adult human brain vasculature transcriptomes (29) showing NOTCH3 is expressed in mural cell lineages (pericytes, smooth muscle cells, fibromyocytes) but not endothelial cell lineages (venous cells, arterial cells, capillary cells, venule cells).