Figure 6 from Hypoxia-Induced Long Noncoding RNA HIF1A-AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer

HIF1A-AS2 is associated with an aggressive clinical stage in HNSCC. A, The expression of HIF1A-AS2 in different clinical stages of patients with GDC-HNSCC (n = 515). The whiskers extend to the minimum and maximum values, with each individual value as a point superimposed on the graph. B, The expression of HIF1A in different clinical stages of patients with GDC-HNSCC (n = 531). The whiskers extend to the minimum and maximum values, with each individual value as a point superimposed on the graph. C, Top: Case No. 21958 represents the patient group with HIF1αhigh/HLA-ABClow /HIF1A-AS2⁺/CD8⁻, whereas Case No. 21454 represents the patient group with HIF1αlow/HLA-ABChigh/HIF1A-AS2⁻/CD8⁺. Positive staining of panCK indicates the tumor cells. The yellow arrows indicate the nuclear HIF1α, whereas the red arrows indicate the membranous HLA-ABC. Scale bar, 100 μm. D, Histogram for illustrating the in situ hybridization HIF1A-AS2 score in the tumor part and the CD8⁺ density of the HNSCC samples. The HIF1A-AS2 staining score was calculated by multiplying the score for staining intensity with the percentage of positive tumor cells. The intensity and the percentage of positive tumor cells were analyzed by QuPath. PanCK staining was used as a marker distinguishing tumors from stroma. Data represent the mean ± SD. *, P < 0.05; ***, P < 0.001.