Figure 1 from Hypoxia-Induced Long Noncoding RNA HIF1A-AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer

Hypoxia-induced HIF1A-AS2 enrichment in HNSCC cells and HNSCC-TEXs. A, Heatmaps for showing the RT-qPCR results of the hypoxia relative lncRNA expression in the cellular (left) and exosomal (right) contents of SAS cells. For establishing the hypoxia-mimic environment, SAS cells were treated with CoCl₂ versus control for 24 hours. Data represent the mean ± SD. n = 3 independent experiments (each experiment contains two technical replicates). B, RT-qPCR for examining the expression of two candidates of hypoxia-induced lncRNAs in the cellular (left) and exosomal (right) contents of SAS and HSC3 cells. For establishing the hypoxia-mimic environment, SAS and HSC3 cells were treated with CoCl₂ versus control for 24 hours. Data represent the mean ± SD. n = 3 independent experiments (each experiment contains two technical replicates). C,HIF1A-AS2 expression in tumor (T, n = 500) versus adjacent normal counterparts (N, n = 44) from TCGA-HNSCC database. D,HIF1A-AS2 expression in tumor (T) versus adjacent normal counterparts (N) of 20 HNSCC paired samples from TVGH (GSE178537). E, Top: Correlation between HIF1A-AS2 and hypoxia signature in the GDC-HNSCC database. Bottom: Correlation between HIF1A-AS2 and hypoxia HIF1A in the GDC-HNSCC database (n = 545). The correlation analysis was performed using the Pearson correlation coefficient. F, The representative confocal image for showing the engulfment of exosomes by SAS cells. The exosomes were derived from hypoxic (Hypo-Exos) or normoxic SAS cells (Nor-Exos). DAPI, 4,6-diamidino-2-phenylindole. Scale bar, 10 μm G, RT-qPCR for examining the HIF1A-AS2 level in SAS cells after treatment with Hypo-Exos versus Nor-Exos. Data represent the mean ± SD. n = 3 independent experiments (each experiment contains two technical replicates). *, P < 0.05; **, P < 0.01; ***, P < 0.001.