Supplementary Figure S8 from Blocking M2-Like Macrophage Polarization Using Decoy Oligodeoxynucleotide-Based Gene Therapy Prevents Immune Evasion for Pancreatic Cancer Treatment

Supplementary Figure S8. Blocking STAT6 and HDAC2 can prevent M2 polarization, maintain M1 polarization, and restore CYBB expression in U937 cells after IL-4 treatment. A, and B, Western blotting analysis for STAT6 and HDAC2 expression in shSTAT6 and shHDAC2 U937 cells after treatment with IL-4 for 1h was performed. C, D, and E, RT-qPCR analysis for M1/M2 markers and CYBB mRNA expression was performed in shSTAT6 and shHDAC2 U937 cells treated with IL-4 for 1h. The bar graphs depict relative mRNA expression. *, p < 0.05; ***, p < 0.001, significant differences between groups. Data represent the mean of 3 independent experiments.