Supplementary Figure S9 from Blocking M2-Like Macrophage Polarization Using Decoy Oligodeoxynucleotide-Based Gene Therapy Prevents Immune Evasion for Pancreatic Cancer Treatment

Supplementary Figure S9. Knocking down STAT6 or HDAC2 in U937 cells prevents pancreatic cancer cells co-culture-induced CYBB downregulation. A, B, and C, RT-qPCR analysis for M1/M2 markers and CYBB in shSTAT6 or shHDAC2 U937 cells co-cultured with PANC-1 cells for 72h. The bar graphs depict relative mRNA expression. ***, p < 0.001, significant differences between groups. Data represent the mean of 3 independent experiments. D, shSTAT6 or shHDAC2 U937 cells were co-cultured with PANC-1 cells for 72h. Cell lysates were subjected to Western blotting for indicated proteins. GAPDH was used as a loading control. The bar graph depicts the ratio of CYBB/GAPDH. **, p < 0.01; ***, p < 0.001, compared with the group without co-culture. Data represent the mean of 3 independent experiments.