Supplementary Figure S11 from Blocking M2-Like Macrophage Polarization Using Decoy Oligodeoxynucleotide-Based Gene Therapy Prevents Immune Evasion for Pancreatic Cancer Treatment

Supplementary Figure S11. STAT6-CYBB ODN-treated macrophages can activate the proliferation of Jurkat cells and αCD3-PBMC cells. A, and B, After scrambled ODN (SC-ODN) and STAT6-CYBB ODN treatment, U937 cells were cocultured with PANC-1 cells for 72h. After coculture with PANC-1 cells, SC-ODN treated macrophages or STAT6-CYBB ODN treated macrophages were cocultured with CFSE-staining Jurkat cells or CFSE-staining αCD3-PBMC cells for 72h. Flow cytometry analysis of cell proliferation in Jurkat cells and αCD3-PBMC was performed. C, and D, The bar graphs indicate the relative percentages of CFSE-labeled Jurkat cells or αCD3-PBMC cells as proliferative index. *, p < 0.05; ***, p < 0.001, compared to the Jurkat cell or αCD3-PBMC cell only group. Data represent the mean of 3 independent experiments.