Supplementary Figure S13 from Blocking M2-Like Macrophage Polarization Using Decoy Oligodeoxynucleotide-Based Gene Therapy Prevents Immune Evasion for Pancreatic Cancer Treatment

Supplementary Figure S13. Treatment with STAT6-CYBB ODNs reduces the proliferation of stromal or epithelial-type tumor cells. A, IF staining for Ki67 and the stromal marker αSMA was performed in KPC tumors from the scrambled ODN, mutated ODN, and STAT6-CYBB ODN-treated mice. Each of the images are representative of a field of immuno-stained cells, taken using a ×20 objective with the Olympus BX53 platform. Each composite image includes the nuclear marker DAPI in blue. B, The Bar graph shows the αSMA (left) and Ki67 (right) positive cells relative to the DAPI-stained nuclei in cells in mouse tumor tissue. ***, p < 0.001, compared to mouse tumors treated with the scrambled-ODN. The random of five fields of 3 random sections from each mouse were used to quantify the positive cells. C, IF staining for Ki67 and the epithelial marker CK7 was performed in KPC tumors from the scrambled ODN, mutated ODN, and STAT6-CYBB ODN-treated mice. Each of the images are representative of a field of immuno-stained cells, taken using a ×20 objective with the Olympus BX53 platform. Each composite image includes the nuclear marker DAPI in blue. D, The Bar graph shows the CK7 (left) and Ki67 (right) positive cells relative to the DAPI-stained nuclei in cells in mouse tumor tissue. ***, p < 0.001, compared to mouse tumors treated with the scrambled-ODN. The random of five fields of 3 random sections from each mouse were used to quantify the positive cells.