Supplementary Figure S4 from Blocking M2-Like Macrophage Polarization Using Decoy Oligodeoxynucleotide-Based Gene Therapy Prevents Immune Evasion for Pancreatic Cancer Treatment

Supplementary Figure S4. H2O2 cannot independently activate JAK/STAT6 phosphorylation. The effect of H2O2 (100 nM) on JAK1, JAK3, STAT6, and CYBB total protein and phosphorylated protein expression was analyzed with Western blotting at indicated time points. GAPDH was used as a loading control. The bar graph depicts the ratio of CYBB/GAPDH. ns, not significant, compared to 0 h. Data represent the mean of 3 independent experiments.