Figure 1 from Netrin-1 and UNC5B Cooperate with Integrins to Mediate YAP-Driven Cytostasis

Outline of CRISPR screen to identify YAP effectors. A, Schematic of the CRISPR screen highlighting timepoints for viral transduction and sample collection. B, Western blot (left) showing expression of ectopic YAP in Y79 cells from three replicates of the CRISPR screen. YAP expression is quantified relative to YAPon NCI-H661 cells. Flow cytometry plot (right) for GFP demonstrating that ∼99% of cells are transduced with the Empty or YAP expression vector. n = 4. C, Criteria used to define hits from the CRISPR screen as (i) having no effect on YAP activity; (ii) being a possible YAP effector; (iii) being a possible YAP inhibitor. YAP-expressing cells (at day 25) were compared with either Input (day 10) or Empty (day 25) cells. sgRNAs that were enriched in YAP-expressing compared with Empty or Input samples represent possible YAP effectors, whereas sgRNAs depleted in YAP-expressing cells represented possible YAP inhibitors.