Figure 2 from Netrin-1 and UNC5B Cooperate with Integrins to Mediate YAP-Driven Cytostasis

A CRISPR screen identifies UNC5B as a YAP effector. A,Z-scores comparing YAP-expressing (day 25) to Empty (day 25) cells for each sgRNA in the CRISPR screen. Select genes are indicated. n = 4. B, TEAD1 knockout rescues YAP-induced cytostasis in Y79 cells (left). Western blot showing TEAD1 knockout efficiency and expression of ectopic YAP (right). *, P < 0.05 compared with sgControl cells, n = 3. C, UNC5B knockout rescues YAP-induced cytostasis in Y79 cells (left). Western blot showing UNC5B knockout efficiency and expression of ectopic YAP (right). ***, P < 0.001 compared with sgControl cells, n = 4. D, RT-qPCR for YAP target genes (AJUBA, AMOTL2, CYR61 and AHNAK) or a control gene (RER1) in control or UNC5B knockout Y79 cells ± ectopic YAP expression. n = 3.