Figure 4 from Activation of Cell-Intrinsic Signaling in CAR-T Cells via a Chimeric IL7R Domain

CCR expression does not diminish CAR T-cell antitumor activity. A, Schematic of the xenograft model. On day 0, NSG mice were injected via tail vein with 1 × 10⁶ CD123⁺ MV-4-11.ffLuc cells. In treatment groups, 2 × 10⁶ T cells were administered on day 7. Cohorts: unmodified (NT), CD28.ζ CAR, and CD28.ζ CAR + IL7Rα CCR-T cells. B, Leukemia proliferation was monitored with BLI, and whole-animal radiance was recorded (photons/second/cm²/sr). Comparison of radiance in each group was performed following log transformation of each measured value. C, Kaplan–Meier survival analysis of MV-4-11 xenografts (n = 9 mice per condition; two independent experiments; median survival: NT: 45.5 days; CAR: 93.5 days; CAR + CCR: undefined; ***, P < 0.001; ****, P < 0.0001). NSG, NOD/SCIDγ; NT, nontransduced.