Abstract Background The aim of this study was to demonstrate a manual semi-isolation specimen bag, and to compare the spillage rate and surgical outcomes between the usage this specimen bag in the process of transumbilical single-port laparoscopy and the conventional technique in laparoscopy for ovarian tumors (less than 12 cm in diameter) presumed to be benign. Methods A retrospective analysis was conducted on 75 patients who underwent laparoscopic resection of ovarian tumors at the People's Hospital of Liuzhou, Guangxi, China, from October 2022 to October 2023. Among them, 40 cases were treated with transumbilical single-port laparoscopy combined with the manual semi-isolation specimen bag (study group), and 35 cases were treated with traditional multi-port laparoscopy (control group). The average total operation time, tumor resection time, time for specimen retrieval after tumor resection, and postoperative recovery were observed in both groups. Results The two groups had similar baseline characteristics. The tumor excision time of control group was shorter than in the study group (42.46 ± 12.77 min vs. 51.88 ± 13.06 min). but the time of specimen removal after tumor excision were significantly shorter in the study group compared to those in the control group(25.98 ± 5.40 min vs. 36.66 ± 15.12 min).However, the average total operation time was similar (101.54 ± 32.16 min vs. 101.28 ± 18.46 min༉. Other surgical outcomes, which were similar between two groups, included length of hospital stay and VAS score at 12h postoperatively. There were no postoperative morbidity in the study group meanwhile 2 in the control group. Conclusion The use of the manual semi-isolation specimen bag in the process of
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