Supplementary Figure S4 from A Senescence-Mimicking (Senomimetic) VEGFR TKI Side Effect Primes Tumor Immune Responses via IFN/STING Signaling

Figure S4: Senomimetic TKI effects induce opposing immune-regulatory molecule expression. (A-C) Gene Expression of antigen presentation molecules following treatment with Ca in (A) 4T1 cells (2µM), (B) EMT6M cells (2µM), and (C) LM2-4 cells (5µM) compared to vehicle treated controls by qRT-PCR (n=3). (D) Gene Expression of antigen presentation molecules in EMT6CaR cells compared to EMT6M by qRT-PCR (n=3). (E) PD-L1 expression in 4T1STING-KD cells after Ax treatment (0.5uM) by qRT-PCR (N=3) (F) Representative histogram and quantification of MHC-I or PD-L1 expression in SM+/SM- gated cell populations in 4T1AxR tumor cells by flow cytometry (n=3). (G) MHC-I and PD-L1 expression in SM+/SM- gated cell populations in EMT6CaR tumor cells by flow cytometry (n=3). Metastatic parental control for EMT6CaR (M); Axitinib-resistant (AxR); Cabozantinib-resistant (CaR); Standard deviation (SD); Median Fluorescent Intensity (MFI); Fold Change (FC). Quantitative data is shown as mean ± SD; * p<0.05; ** p<0.01; *** p<0.001; **** p < 0.0001