Supplementary Figure S5 from A Senescence-Mimicking (Senomimetic) VEGFR TKI Side Effect Primes Tumor Immune Responses via IFN/STING Signaling

Supplementary Figure S5: Extended data from Fig 5 - CD8 T-cell stimulating effects of SM+ cells off-set the immune-suppressive secretory effects. (A-B) Log2FC of %Divided, %GRMB+, or %IFNγ+ gated CD8+ stimulated splenocytes following treatment with (A) CM derived from EMT6CaR tumor cells or (B) co-cultured with EMT6CaR compared to respective parental controls evaluated by flow cytometry (n=3-4). (C-D) %Divided, %GRMB+, or %IFNγ+ gated CD8+ stimulated splenocytes following treatment with (C) CM derived from 4T1AxR tumor cells or (D) co-cultured with 4T1AxR compared to respective parental controls evaluated by flow cytometry (n=3). (E-F) %Divided, %GRMB+, or %IFNγ + gated CD8+ stimulated splenocytes following treatment with (E) CM derived from EMT6CaR tumor cells or (F) co-cultured with EMT6CaR compared to parental controls evaluated by flow cytometry (n=3-4). (G-H) Log2FC of %Divided, %GRMB+, or % IFNγ + gated CD8+ stimulated splenocytes following treatment with (G) CM derived from EMT6CaR SM+ tumor cells or (H) co-cultured with EMT6CaR SM+ compared to SM- controls evaluated by flow cytometry (n=3-4). (I-J) %Divided, %GRMB+, or %IFNγ+ gated CD8+ stimulated splenocytes following treatment with (I) CM derived from 4T1AxR SM+ tumor cells or (J) co-cultured with 4T1AxR SM+ compared to SM- controls evaluated by flow cytometry (n=3-9). (K-L) %Divided, %GRMB+, or %IFNy+ gated CD8+ stimulated splenocytes following treatment with (K) CM derived from EMT6CaR SM+ tumor cells or (L) co-cultured with EMT6CaR SM+ compared to SM- controls evaluated by flow cytometry (n=3-4). Parental (P); Metastatic parental control for EMT6CaR (M); Axitinib-resistant (AxR); Cabozantinib-resistant (CaR); Senescence-marker (SM); Granzyme B (GRMB); Interferon gamma (IFNγ); Conditioned media (CM); Fold Change (FC); Standard Deviation (SD); Normalized (Norm.); Quantitative data shown as mean ± SD. * p<0.05; ** p<0.01; ***n p<0.001; **** p<0.0001.