The aim – to investigate metabolic activity changes of the microbiota of the large intestinein rats with experimental diabetes.Material and methods. Diabetes mellitus (DM) was modeled by a single intraperitonealadministration of streptozotocin (Sigma, USA, 60 mg/kg body weight) to two-month-oldmale rats. The duration of diabetes is four months. Colon microbiota metabolites (acetic,propionic, oleic, lactic, oxalic- acetic, ά-ketoglutaric, phenylpropionic acids, n-cresol,skatole, indole) were studied by gas-liquid chromatography. Biogenic amines (methylamine,histamine, serotonin) were studied by the method of highly effective liquid chromatography.The results. Determination of metabolic indicators of microbiocenosis in diabetesrevealed a significant decrease in the content of carboxylic acids: acetic, propionicbutyric and lactic, which is consistent with a decrease in the number of anaerobicintestinal microflora (lacto- and bifidobacteria, bacteroides). A significant decrease in thelevels of ά-ketoglutaric and oxalic-acetic acids was established. The profile of aromaticcompounds was characterized by a significant decrease in the content of n-cresol, skatole,phenylpropionic acid against a background of an increase in the level of indole in relationto the corresponding indicators in the control group. In addition, in rats with four-monthdiabetes, an increased secretion of methylamine was determined against the backgroundof a reduced content of histamine and serotonin.Conclusions. According to the profile of the microflora metabolites of the colon in ratswith DM, intestinal dysbacteriosis is present, characterized by a decrease in the metabolicactivity of physiologically useful anaerobic autochthonous obligate bifidobacteria andlactobacteria and the accumulation of certain toxic metabolic products.
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