Collection, Generation and Identification of Bone Marrow Mesenchymal Stem Cells (Bm-Mscs), Taken from Mice

Mesenchymal stem cells are multipotent cells capable of secreting various factors, including macrophage colony-stimulating factor, transforming growth factor-beta, vascular endothelial growth factor, insulin-like growth factor-1, and exosomes. These cells can differentiate into various cell types and migrate to damaged areas, making them valuable in both preclinical and clinical research. BM-MSCs have potential therapeutic applications for conditions such as heart and immune system disorders, multiple sclerosis, Alzheimer’s disease, spinal cord injuries, bone fractures, and cartilage damage. This study focused on isolating BM-MSCs from mice, producing, and identifying them. For identification, flow cytometry was used. Cells were removed from petri dishes, treated with 1 ml of staining buffer, and incubated with an antibody or isotype control. The analysis was conducted using a BD Facs Aria III instrument. The results showed that the BM-MSCs had the following surface marker expression levels: CD45- at 99.8%, CD105+ at 97.6%, CD29+ at 88.3%, and SCA-1+ at 93%. Overall, MSC research is a prominent and evolving field both nationally and globally, offering significant opportunities for preclinical and clinical applications. Thus, the processes of harvesting, producing, and identifying stem cells are crucial.