An analytical method was developed using high performance liquid chromatography with refractive index detection (HPLC-RID) to quantify the concentration of ethylene glycol and 1,4-butanediol in aqueous samples. This list is non-exhaustive as this isocratic method with an acid modified mobile phase is compatible with analysis of many alcohols, small acids, and sugars. This method utilizes a Bio-Rad Aminex HPX-87H Ion Exclusion Column to provide chromatographic separation.
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Alt et al. (2024) studied this question.
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