Figure 5 from Spatial Landscape of Malignant Pleural and Peritoneal Mesothelioma Tumor Immune Microenvironments

Analysis of cell types in MPM BAP1-high and BAP1-low tumor groups. A, Correlation between mean CD8⁺ (cytotoxic T-cell) and CD11c⁺ (DC) intensities and mean BAP1 expression levels. Dots are color-coded by histologic subtypes. B, Ridge density plot illustrating tumor and CD8⁺ T cell contact scores among BAP1-high and BAP1-low pan-CK–enriched cores (cores with CK percent>20%). Density values >0.6 quantile and <0.4 quantile were used as thresholds to categorize cores as BAP1-high and BAP1-low, respectively. C and D, Segmented images show increased interaction between cancer cells and CD8⁺ T cells in BAP1-low MPM (D) compared with BAP1-high MPM (C). Top, TMA core sections stained with H&E, in which BAP1 is represented in Opal 690 (red); NF2 in Opal 780 (orange); MTAP in Opal 620 (green); and LAG3 in Opal 570 (magenta). Scale bars, 100 μm, 10× original magnification. Middle, Zoomed-in area from the boxed segment in the top, stained with DAPI for DNA (cyan); Opal 480 for CD8⁺ T cells (yellow); and Opal 780 for pan-CK cells (blue). Scale bars, 10 μm, 100× original magnification. Bottom, Composite images. Scale bars, 30 μm, 30× original magnification.