Supplementary Figure S4 from Missense Mutations in Myc Box I Influence Nucleocytoplasmic Transport to Promote Leukemogenesis

Supplementary Figure 4. (A) Histogram plot showing the viability (as measured by Acridine Orange/Propidium Iodine stain read on a Cellometer instrument) of control cells, MYCWT, MYCP59Q and MYCT58Nexpressing cells at before (time 0) and 8 hours after treatment with DRB. None of the difference are statistically significant. (B) Schematic overview of the nascent proteomic analysis experimental setup. (C) Volcano plots of the fold changes in spectral counts of MYC overexpressing (doxycycline treated) vs. control (vehicle treated) cells (Supplementary Data S7). Black dashed lines indicate significance (ANOVA, Dox vs Vehicle, fold changes ± 2, adjp<0.05) (D-E) Volcano plots showing the trends in spectral counts fold changes (x-axis) over 30 minutes of OPP nascent polypeptide labeling for cluster 3 genes (D) and for the genes in figure 4A (E). Comparisons are MBI MYCMUT*Dox vs MYCWT*Dox. Red dashed lines indicate significance.