Supplementary Figure S7 from Missense Mutations in Myc Box I Influence Nucleocytoplasmic Transport to Promote Leukemogenesis

Supplementary Figure 7. (A) UMAP projection of 36085 cells from MycT58N/+ and Myc+/+ mice colored by genotype, as indicated in the legend. (B) UMAP projection of 36085, split by genotype and colored by the combined expression of Mpo (Myeloperoxidase) and Elane (Elastase, Neutrophil Expressed) transcripts. In yellow are GMP cells, that strongly express both transcripts. (C) UMAP projection of 36085 cells split by genotype and colored by the combined expression of Cd177 and Ngp (neutrophil granule protein). In yellow are granulocytopoietic progenitors, that express high levels of both transcripts. (D) Trajectory analysis of 36085 showing the temporo-spatial expression of Myc (cells that express Myc are in shades of red). (E) Venn diagram showing the percentage overlap in scRNA-seq DEGs between the overexpression and the knockin mouse model. Comparisons are: Empty vector*GMP vs. MycT58N*GMP and MycT58N/+* GMP vs. Myc+/+*GMP for the overexpression and the knockin, respectively. 55% of the DEGs (8334 genes) are shared between the two models. (F) Schematic summary of the nucleocytoplasmic transport components’ transcripts that are upregulated >2 folds in MycT58N/+ versus Myc+/+(created with BioRender.com) (G) Enrichment plots (top) and heatmaps of the leading-edge genes in each pathway (bottom), showing the differential enrichment on Ribosome Biogenesis, Pyrimidine metabolism and Pluripotency pathways in MycT58N/+ GMP-like cells. (H) Enrichment plots (top) and heatmaps of the leading-edge genes in each pathway (bottom), showing the enrichment of Hematopoietic cell lineage and Immune pathways in Myc+/+GMP-like cells.