Supplementary Figure S2 from Missense Mutations in Myc Box I Influence Nucleocytoplasmic Transport to Promote Leukemogenesis

Supplementary Figure 2. (A) Colony forming ability (progenitor cell content) per equal number of Linlow GFP+ cells in HSPCs transduced with EV,MycWT,MycP59Q, MycT58N. 10,000 GFP+ cells per genotype were plated in M3434 media and enumerated 7-10 days later. 10,000 cells recovered from each round were plated again and enumerated 7-10 days later for 3 subsequent rounds. Bar graphs represent average and standard deviation. One-way ANOVA, n = 3 replicate per genotype, *p<0.05, ***p<0.001. (B) Boxplot representing AUCell (y-axis) expression for the 247 genes of cluster 3 in RNA sequencing from AML cells with MYC mutations vs. AML cells without MYC mutations ***FDR<0.001. (C) Heatmap representing the DEGs at bulk RNA sequencing between GFP+ GMP EV control cells vs. Mycoverexpressing cells (Supplementary Data S5). (D) Scatterplot of all terms in the KEGG_2019_Mouse gene set library. Genes upregulated and downregulated by in the bulk RNA sequencing analysis of Myc overexpressing cells are highlighted by red and blue circles respectively