Theoretical and experimental evaluation of the test and retest reliability of single- and multi-delay 3D pCASL

The goal of this study is to evaluate the quantification accuracy and measurement reliability of single- and multi-PLD pCASL via both simulation and in-vivo test and retest data. CBF calculated by single-delay ASL achieved highest test and retest reliability as compared to 5-PLD ASL. However, CBF values may be under-estimated due to prolonged ATT. 5-PLD ASL can be used to quantify ATT and CBF simultaneously with slightly reduced test and retest reliability. Model-fitting approach is more preferrable than the weighted-delay approach in terms of both quantification error and reliability.