1H-MRS human skeletal muscle quantification: a repeatability analysis

1H-MRS of skeletal muscle presents challenges due to the highly structured arrangement of muscle fibres and their orientation to B0, complicating metabolite visibility and quantification. This study aimed to assess the repeatability of 1H-MRS in the anterior tibialis muscle in healthy volunteers by acquiring multiple spectra during and within different scanning sessions. A robust method for positioning of the voxel-of-interest was used to ensure reproducible acquisition. Data analysis was done using LCModel. Statistical analysis showed no significant difference in metabolite ratios against water across sessions, suggesting good repeatability of 1H-MRS of skeletal muscle.