1H NMR of downfield metabolites in swine heart after myocardial infarction

Growing interest into in vivo 1H MRS of downfield metabolites (>4.7 ppm) has necessitated greater understanding of the metabolite contributions of peaks found in the downfield. 1H NMR provides high-SNR, high-spectral resolution data that can aid in the identification of metabolite resonances. We collected downfield NMR spectra at 700 MHz from infarct and remote myocardium extract of pig hearts after myocardial infarction. We were able to assign peaks to NAD+, NADH, ATP, and AMP in the downfield region and quantified these metabolites in infarct and remote myocardium.