Development for hyperpolarized [1-13C]alpha-ketoglutarate MRI for metabolic imaging of normal volunteers and mutant IDH glioma patients

Hyperpolarized [1-13C]alpha-ketoglutarate (aKG) is a metabolic probe that can provide direct access to the metabolic pathway for glutamate production or 2-HG production in altered alpha-ketoglutarate metabolism via IDH mutations. With its potential to serve as a biomarker of IDH1 mutational status in glioma and a measure of aKG metabolism in normal healthy subjects, we developed a new chemistry preparation and standard operating procedure (SOP) for on-site production of hyperpolarized GMP [1-13C]alpha-ketoglutarate and characterized the probe to investigate the feasibility of this probe in clinical setting. Following process qualification trials with HP [1-13C]aKG, an FDA IND application was prepared and submitted.