Figure 3 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors

Mass spectrometry analysis of the tumor-derived lipid rafts. A, Designation of the brain tumor regions on postcontrast T1-weighted image for obtaining tumor samples from necrotic, tumor, and peritumoral regions. B, Representative confocal microscopy images of the brain tumor samples obtained from three regions and stained for mHsp70 (green) and DAPI (blue) for nuclei. Scale bars, 100 μm. C–E, Analysis of the mass spectrometry data from isolated lipid rafts. Protein functional groups identified using the STRING database in the proteome of lipid rafts from three tumor zones (Supplementary Fig. S7). C, A subnetwork of highly membrane-associated proteins isolated from the tumor-derived lipid raft and D, normal tissue-derived lipid raft STRING PPI proteome network, separated into clusters using the Markov cluster algorithm method. Color mapping (light to dark) was done to indicate the nodes belonging to the cytoskeletal compartment, the size of the node corresponding to the protein abundance index obtained from mass spectrometry data. E, The cluster containing highly plasma membrane-associated proteins contains simultaneously heat shock proteins, small GTPases, and cytoskeletal proteins.