Figure 4 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors

Motility analysis of sorted C6, T98G, and U251 cell lines by the level of mHsp70 expression. A, Representative scheme of mHsp70 expression analysis and FACS for subsequent determination of cell migration characteristics. B, Confocal microscopy images of C6, T98G, and U251. DAPI was applied for nucleus staining (blue). FITC-IgG1 and FITC-anti-Hsp70 antibody was used for detecting mHsp70 on the plasma membrane (green). Scale bars, 50 μm. C, Mean fluorescence intensity of FITC-anti-Hsp70 antibody in arbitrary units measured by flow cytometry (n = 4). D, Example of tracks rose plots in motility analysis. E, C6, T98G, and U251 cell tracks violin plots. F, Phase contrast live images of wound healing assay of subpopulations with different mHsp70 expression and plots of wound area vs. healing time. Scale bars for images, 400 μm.