Figure 6 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors

Inhibition of mHsp70 results in decreased motility of patient-derived brain tumor cells. A, The process of isolating the primary human GBM cell line from postoperative material, obtaining confocal images, shooting on a live-cell imaging platform with the addition of inhibitors, and subsequent training and implementation of an AI model for tracking highly invasive cells. B and C, Representative confocal microscopy images of the patient-derived tumor cell lines stained for mHsp70 (green), SOX2 (magenta), and nestin (magenta). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. D, CD133 expression in patient-derived tumor cells stained by PE-anti-CD133 antibody (as measured by flow cytometry). E, Comparison of mean speed and track straightness on various matrices (PL, MG, and FN) of patient-derived high-speed brain tumor cells treated with 1-μmol/L PES or 50-nmol/L JG98 (ntracks ≥ 294). Nontreated cells were used as a control. Data are presented as median ± 95% CI. Significant differences identified by the Wilcoxon test are shown as *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.