Figure 2 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors

Analysis of the mHsp70-positive tumor cell motility in brain tumors. A, Representative confocal microscopy images from 68 hours of time-lapse recording of the tumor sample (patient with GAA) stained for mHsp70 (green). Scale bars, 500 μm. The numbers show the time in the format days:hours:minutes:seconds. B, Histograms and density plots of values characterizing tracks of motile cells emerging from a tumor sample: max speed (μm/hour), mean speed (μm/hour), sinuosity, and straightness. C, Staggered analysis plots show the changes in cells’ mean speed during 68 hours; four cells with different motility patterns are shown. D, MSD plots on tracks (ncells = 50) of 68 hours tumor cell motility in comparison with a simulated random walk. E–H, Migration characteristics of mHsp70⁺ and patient with mHsp70⁻ IBD explant cell subpopulations. E, Microscopy images of explant. From top to bottom: brightfield image with marked tracks of mHsp70⁻ and mHsp70⁺ cells, brightfield image, and corresponding image of mHsp70 fluorescence channel. F, Zoomed in samples of microscopy images. C, Rose plots of patient with IBD explant cell subpopulations. G, Comparison of mean speed, straightness, and sinuosity of subpopulations (ntracks ≥ 51). H, Comparison of the mean speed of nontreated and treated subpopulations with 20 μmol/L PES (ntracks ≥ 59). Data are presented as median ± 95% CI. Significant differences identified by the Wilcoxon test are shown as *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.