Figure 5 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors

Application of Hsp70 inhibitors decreases the brain tumor cell migratory activity. A–C, mHsp70 expression in nontreated C6, T98G, U251 cell lines and cells incubated with 1 μmol/L PES or 50 nmol/L JG98 inhibitors. A, Confocal microscopy images of C6, T98G, U251. DAPI was applied for nucleus staining (blue). FITC-anti-Hsp70 antibody was used for detecting mHsp70 on the plasma membrane (green). Scale bars, 50 μm. B, Mean signal intensity of FITC-anti-Hsp70 antibody in nontreated C6, T98G, U251 cell lines and cells incubated with 1 μmol/L PES or 50 nmol/L JG98 inhibitors observed by confocal microscopy (nimages ≥ 20). C, Mean fluorescence intensity of FITC-anti-Hsp70 antibody in arbitrary units measured by flow cytometry. D, Comparison of mean speed, straightness, and sinuosity of sorted nontreated and treated C6, T98G, and U251 cells with 1 μmol/L PES or 50 nmol/L JG98 (ntracks ≥ 46). Data are presented as median ± 95% CI. Significant differences identified by the Wilcoxon test are shown as *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.