Figure 2 from p300 KAT Regulates SOX10 Stability and Function in Human Melanoma

SOX10 signaling requires p300 KAT activity. (A) A panel of melanoma cell lines were treated with DMSO (D) or 5 µmol/L A-485 (A) for 24 hours. SOX10, MITF, and DCT protein levels were assessed via immunoblotting. B, A panel of EP300/SOX10 co-amplified melanoma cell lines were treated with DMSO (D) or 5 µmol/L A-485 (A) for 24 hours. MITF and DCT expressions were assessed via RT-qPCR. C,MITF, SCD, and MYC expressions were downregulated by SOX10 KD in A375 cells. D, A panel of melanoma cell lines without EP300/SOX10 co-amplifications were treated with DMSO (D) or 5 µmol/L A-485 (A) for 24 hours. MITF, SCD, and MYC expressions were assessed via RT-qPCR. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.