Figure 3 from p300 KAT Regulates SOX10 Stability and Function in Human Melanoma

A-485 induces proteasomal degradation of SOX10. A, A panel of melanoma cell lines were treated with DMSO (D) or 5 µmol/L A-485 (A) for 24 hours. SOX10 expression was assessed via RT-qPCR. B–D, IPC-298, CO79, and A375 cells were treated with 50 µg/mL CHX and/or 5 µmol/L MG-132 for the indicated time. SOX10 protein levels were assessed via immunoblotting. E–G, IPC-298, CO79, and A375 cells were treated with DMSO, 5 µmol/L A-485, 5 µmol/L MG-132, or their combination for 16 hours. SOX10 protein levels were assessed via immunoblotting. *, P < 0.05.