Figure 4 from p300 KAT Regulates SOX10 Stability and Function in Human Melanoma

A-485 treatment potently downregulates SOX10 target genes in melanoma cells. A–C, IPC-298, CO79, and A375 cells were treated with 5 µmol/L A-485 for 24 hours, and RNA-seq was performed. Volcano plots of DEGs in each cell line are depicted (DEGs defined as FC > |2| and P < 0.05). D, The top five GO biological process pathways are shown for downregulated genes in each cell line. E, A heatmap of DEGs resulting from SOX10 KD in A375 cells is shown (data from GSE50535). F, The top five GO biological process pathways are shown for SOX10-activated genes (i.e., genes downregulated by SOX10 KD). G, A-485 downregulates GO biological process pathways that are activated by SOX10 in IPC-298, CO79, and A375 cells. H, Volcano plots of SOX10-activated genes differentially expressed due to A-485 treatment for IPC-298, CO79, and A375 cells. I–K, GO biological process pathways are shown for SOX10-activated genes that are downregulated by A-485.