Supplementary Figure S2 from Overexpression of an Engineered SERPINB9 Enhances Allogeneic T-cell Persistence and Efficacy

Supplementary Figure S2. Mixed lymphocyte reaction assay with TCRαβKO ATCs. A, Expression of TCRαβ on CD3 wild-type (WT) T cells or T cells after TCRαβKO gene modification using Crispr gene editing measured by flow cytometry analysis. B, Expression of different variants of SB9 on TCRαβKO ATCs measured by intracellular flow cytometry analysis of one representative donor. C, Relative geometric mean fluorescence intensity (MFI) of different variants of SB9, normalized to SB9 expression in NT sample. The bars denote median ± SD (n = 3 donors). D, Cell counts, by flow cytometric analysis, of ‘recipient’ CD3-CD56+ NK cells that were in co-culture with ‘donor’ TCRαβKO ATCs in a mixed-lymphocyte reaction study, from the same representative ‘donor’-‘recipient’ pair as shown in Fig. 1C–E.