A Study on Inducible Clindamycin Resistance Among Mrsa Isolates From the Clinical Samples in a Tertiary Care Hospital , Warangal.

Introduction: Staphylococcus aureus is one of the most common human pathogens capable of causing a wide range of infections, which are hospital acquired as well as community acquired. Increasing incidence of methicillin resistance among staphylococci has led to usage of macrolide-lincosamide-streptogramin B (MLSB) antibiotics to treat S.aureus infections, with clindamycin being the preferable agent owing to its excellent pharmacokinetic properties. Inducible clindamycin resistance may lead to therapeutic failure. Aim And Objectives: To detect the prevalence of constitutive and inducible clindamycin resistance in clinical isolates of methicillin resistance staphylococcus aureus (MRSA) by Dtest method to improve the clinical outcomes in patients. Materials And Methods: This is a prospective study done in a total of 120 MRSA isolates obtained from different clinical samples. Methicillin resistance was detected using cefoxitin disc-diffusion method. D-test will be performed by Kirby-bauer disc diffusion method using Mueller-Hinton agar (MHA) according to standard CLSI guidelines. Results: Out of 120 MRSA isolates, 108 shows erythromycin resistance and 96 shows clindamycin sensitive. Among erythromycin resistant isolates 11% showed constitutive resistance (MLSB ), 26% showed inducible clindamycin resistance (MLSB ) 63% showed MS phenotype. The frequency of C i Conclusion: constitutive and inducible clindamycin resistance in MRSA isolates emphasizes that D-test is very important and it must be included as routine antimicrobial sensitivity test in diagnostic laboratories.