Development and Validation of a Relative Blood Flow Change Measurement Algorithm Using Near-Infrared Light

The purpose of this study is to develop a near-infrared blood flow measurement algorithm and verify its performance. The gradient value of the measurement point was determined by using LED lights with wavelengths of 760[Formula: see text]nm and 850[Formula: see text]nm. This process involved measuring the gradient value for a significant number of regularly spaced cubic pixels centered around the chosen point. The measured gradient values for each pixel were then averaged to derive the final gradient value. Based on the measurement of the gradation value, when the concentration ratio of blood was altered by 20% between 0% and 100%, the gradation values at 760[Formula: see text]nm and 850[Formula: see text]nm changed significantly (more than [Formula: see text]) in all cases. This study validated the efficacy of a self-developed near-infrared light system for detecting changes in blood flow. The ability to measure alterations in blood flow can be instrumental in predicting peripheral vascular disease (PVD) due to compromised circulatory conditions.