Evaluating the droplet digital PCR and direct Sanger sequencing for the detection of the BRAFV600E mutation in papillary thyroid carcinoma

• DdPCR has the ability to detect low-frequency BRAFV600E mutations. • DdPCR provides quantitative precession that is it provides absolute quantification of mutant alleles. • Sanger sequencing has the ability to identify quantitative mutation that can be either novel or known. • Combining ddPCR and Sanger sequencing helps to enhance the diagnostic accuracy.