Figure 3 from Claudin-4 Modulates Autophagy via SLC1A5/LAT1 as a Mechanism to Regulate Micronuclei

In vivo association between indicators of autophagy and genomic instability during CMP treatment. Ascites was obtained from tumor-bearing mice treated or not with CMP (4 mg/kg) and prepared in histogel, followed paraffin embedding and multispectral staining (eight proteins plus DAPI). Afterward, a phenotypic characterization was performed. A, Frequency of cells obtained from ascites samples at the end of the study (30 days) showing individual markers as well as cells positive for LC3 A/B and pSTING, and LC3 A/B, pSTING, and pTBK1. B, Representative multispectral IF images (all markers used are indicated in the blue-light box) are shown, where a region of interest (white square) is amplified to show specific markers (white arrow are highlighting the overlay of nuclei and the marker of autophagy, LC3 A/B). (Multiple t test; significance; P < 0.05). Graphs show mean and SEM, scale bar, 50 µm.