Supplementary Figure 1 from Ultrasensitive Response Explains the Benefit of Combination Chemotherapy Despite Drug Antagonism

Supplemental Figure 1. Reproducibility and dynamic range of in vitro experiments. A) Consistency of measured isobolograms among biological replicates (independently propagated cultures; left and right columns) and among technical replicates (different microtiter plates; top and bottom rows). Cell line = MTA, n = 1 per plot here, with each of 4 plots averaged to produce isobolograms in Supplemental Figure 2. B) Dynamic range of CLARIOstar microplate reader is linear over 5 orders of magnitude in cell density (10¹ to 10⁵ Ki-JK cells per well). Red shaded region represents 95% confidence interval of population mean (n = 16). C) Reproducibility of two replicate 15-day treatments conducted two weeks apart from one another. Untreated cells are compared to the two-halves dosing scheme where 12.5 nM doxorubicin was administered for 72 hours on Days 0 and 6 (as shown in Figure 1). Cell line = MTA, n=6, error bars represent 95% confidence interval.