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July 2, 2024

JAX-Sen: Mouse pancreas dissociation for single-cell RNA sequencing v1

Ramalakshmi Ramasamy Juliana Alcoforado Diniz Patrick J. Fleming Jessica Garofalo Dylan Baker Paul Robson

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Abstract

These samples are part of the JAX-Sen project in the SenNeT Consortium. We aim to study and characterize senescence in the C57Bl/6 mouse pancreas. We compare young (6 months old) and old (24 months old) mouse pancreas using scRNAsequencing. This protocol describes the single-cell dissociation of the pancreas before library preparation and sequencing.

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Cite This Study

Ramasamy et al. (2024) studied this question.

synapsesocial.com/papers/68e61c93b6db6435875aeabe https://doi.org/10.17504/protocols.io.ewov19zzylr2/v1

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Keep reading

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1JAX-Sen: Collection and shipment of specimen for single-cell RNA sequencing (scRNA-Seq) v22024
2JAX-Sen: Collection and shipment of specimen for single-nuclei RNA sequencing (snRNA-Seq) v22024
3Collection and shipment of specimen for single-cell RNA sequencing (scRNA-Seq) v12024
4JAX-Sen: Single Cell Dissociation of Mouse Placenta v12025
5Collection and shipment of specimen for single-nuclei RNA sequencing (snRNA-Seq) v12024