This protocol demonstrates single cell suspension acquisition from mouse placenta, highlighting enzymatic digestions and timepoints across multiple strains.
This protocol outlines the process of acquiring a single cell suspension prior to submission for single cell RNA sequencing. Whole mouse placentas were collected from male and female fetuses across 8 mouse strains (WSB, A/J, NOD, NZO, 129S1, PWK, CAST, C57Bl/6). Placentas were collected at 4 different timepoints for the C57Bl/6 strain (E9.5, E11.5, E13.5, and E17.5) and 1 timepoint (E13.5) for the other 7 strains. The whole mouse placenta is minced manually, undergoes two enzymatic digestions and ACK Lysis, and gets carefully filtered. Cells are then sorted to enrich for live cells and eliminate cellular debris.
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Ramasamy et al. (2025) studied this question.
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