This protocol details bulk rna extraction from mouse placenta samples using bead homogenization, indicating methodological improvements for rna sequencing.
This protocol outlines the process of tissue homogenization and bulk RNA extraction that occurs prior to submission for bulk RNA sequencing. Whole mouse placentas were collected from male (n=4) and female (n=4) fetuses across 8 mouse strains (WSB, A/J, NOD, NZO, 129S1, PWK, CAST, C57Bl/6). Placentas were collected at 4 different timepoints for the C57Bl/6 strain (E9.5, E11.5, E13.5, and E17.5) and 1 timepoint (E13.5) for the other 7 strains for a total of 88 placenta samples. Tissue disruption of the whole mouse placenta is accomplished via the BeadBug 6 homogenizer, after which an aliquot of homogenized lysate is used with the QIAGEN RNeasy Plus Mini kit to extract bulk RNA.
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Ramasamy et al. (2025) studied this question.
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