NGS library preparation using Ovation RNA-Seq System V2 (M01206 v9) and Ovation Ultralow System V2 (M01437 v2) for animal tissue samples v1

This protocol is used for successful NGS library preparation from total RNA of animal tissue samples. With this method we obtained full-length paramyxovirus genomes using the Illumina sequencing platform. It works most efficiently on RNA viruses possessing a polyA tail, but it can also be used for sequencing any other RNA viral sequences originating from tissue samples.